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Product Name
MYPT1 antibody
- Documents
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Description
MYPT1 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human gliomas tissue, human skeletal muscle tissue. Positive IF detected in HeLa cells. Positive IP detected in HEK-293 cells. Positive WB detected in HeLa cells, HEK-293 cells. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MBS antibody; MYPT1 antibody; PPP1R12A antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYPT1 recombinant protein (Accession Number: NM_002480). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113001(MYPT1 antibody) at dilution of 1:3000
Immunohistochemical of paraffin-embedded human heart using Catalog No:113001(MYPT1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:113001(MYPT1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MYPT1 (IP:Catalog No:113001, 4ug; Detection:Catalog No:113001 1:500) with HEK-293 cells lysate 1200ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:113001(MYPT1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PPP1R12A represents protein phosphatase 1, regulatory (inhibitor) subunit 12A
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"