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Product Name
Myosin 2a antibody
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Description
Myosin 2a Mouse Monoclonal antibody. Positive WB detected in human skeletal muscle tissue. Positive IHC detected in human skeletal muscle tissue. Observed molecular weight by Western-blot: 200 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
MYH2 antibody; MYH2A antibody; MYHas8 antibody; MyHC 2A antibody; MyHC IIa antibody; MYHSA2 antibody; Myosin 2 antibody; Myosin 2a antibody; Myosin heavy chain 2 antibody; Myosin heavy chain 2a antibody; Myosin heavy chain IIa antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_017534). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:107318(Myosin 2a Antibody) at dilution of 1:4000
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:107318(Myosin 2a Antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:107318(Myosin 2a Antibody) at dilution of 1:100 (under 40x lens)
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Background
MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins.
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