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Product Name
Myoferlin antibody
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Description
Myoferlin Rabbit Polyclonal antibody. Positive WB detected in A549 cells. Positive IF detected in Hela cells. Positive IHC detected in human placenta tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 235 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human; other species not tested.
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Alternative names
Fer 1 like protein 3 antibody; FER1L3 antibody; FLJ36571 antibody; FLJ90777 antibody; KIAA1207 antibody; MYOF antibody; Myoferlin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_133337). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:112991(MYOF antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human placenta using Catalog No:112991(MYOF antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:112991(MYOF antibody) at dilution of 1:100 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug MYOF antibody (Catalog No:112991, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunofluorescent analysis of Hela cells, using MYOF antibody Catalog No:112991 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
MYOF, also named as FER1L3 and KIAA1207, belongs to the ferlin family. MYOF is calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is involved in endocytic recycling. MYOF implicated in VEGF signal transduction by regulating the levels of the receptor KDR. The antibody can recognize all the 8 isoforms of MYOF.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"