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Product Name
MYL3 antibody
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Description
MYL3 Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse heart tissue, human heart tissue, mouse skeletal muscle tissue. Positive IHC detected in human heart tissue, human lung cancer tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 22-27 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cardiac myosin light chain 1 antibody; CMH8 antibody; CMLC1 antibody; MLC1SB antibody; MLC1V antibody; MYL3 antibody; Myosin light chain 3 antibody; VLC1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYL3 recombinant protein (Accession Number: NM_001406937). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:2000-1:20000
IHC: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112943(MYL3 antibody) at dilution of 1:1000
IP Result of anti-MYL3 (IP:Catalog No:112943, 3ug; Detection:Catalog No:112943 1:7000) with mouse heart tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:112943(MYL3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:112943(MYL3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)
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References
- Addona TA, Shi X, Keshishian H. A pipeline that integrates the discovery and verification of plasma protein biomarkers reveals candidate markers for cardiovascular disease. Nature biotechnology. 29(7):635-43. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"