Product Name

MYL3 antibody

Documents

Description

MYL3 Mouse Monoclonal antibody. Positive WB detected in human skeletal muscle tissue, rat heart tissue. Positive IHC detected in human heart tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 22-25 kDa

Tested applications

ELISA, IHC, WB

Species reactivity

Human, Rat; other species not tested.

Alternative names

Cardiac myosin light chain 1 antibody; CMH8 antibody; CMLC1 antibody; MLC1SB antibody; MLC1V antibody; MYL3 antibody; Myosin light chain 3 antibody; VLC1 antibody

Isotype

Mouse IgG1

Preparation

This antibody was obtained by immunization of MYL3 recombinant protein (Accession Number: NM_001406937). Purification method: Protein G purified.

Clonality

Monoclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:5000-1:50000

IHC: 1:20-1:200

Validations

human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:107315(MYL3 Antibody) at dilution of 1:20000

Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:107315(MYL3 Antibody) at dilution of 1:600 (under 10x lens).

Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:107315(MYL3 Antibody) at dilution of 1:600 (under 40x lens).

Background

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

MYL3 antibody

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