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Product Name
MYL12B antibody
- Documents
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Description
MYL12B Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue, mouse heart tissue. Positive IHC detected in human colon cancer. Observed molecular weight by Western-blot: 20kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
MYL12B antibody; MLC B antibody; MLC 2A antibody; SHUJUN 1 antibody; MLC 2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYL12B recombinant protein (Accession Number: NM_033546). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:112941(MYL12B antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112941(MYL12B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112941(MYL12B antibody) at dilution of 1:100 (under 40x lens)
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Background
MYL12B is a regulatory subunit of myosin and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. There are two groups of residues on the MYL12B that are phosphorylated by distinct kinases and have contrasting effects on myosin II biophysical properties.Phosphorylation at Thr18/Ser19 essentially “activates” the myosin molecule to produce force. The second group of phosphorylated residues is at the N-terminus of the MYL12B at Ser1, Ser2 and Thr9 and causes inhibitory effect on myosin activity.(22136066)
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References
- Breckenridge MT, Dulyaninova NG, Egelhoff TT. Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells. Molecular biology of the cell. 20(1):338-47. 2009.
- Beach JR, Licate LS, Crish JF, Egelhoff TT. Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells. BMC cell biology. 12:52. 2011.
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