MYH7-specific antibody

Cat.#: 112936

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Product Information

  • Product Name
    MYH7-specific antibody
  • Documents
  • Description
    MYH7-specific Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue. Positive IP detected in mouse heart tissue. Positive IHC detected in mouse heart tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 200-220 kDa
  • Tested applications
    ELISA, IHC, WB, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CMD1S antibody; CMH1 antibody; MPD1 antibody; MYH7 antibody; MyHC beta antibody; MyHC slow antibody; MYHCB antibody; Myosin 7 antibody; Myosin heavy chain 7 antibody; SPMD antibody; SPMM antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_000257). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112936(MYH7 Antibody) at dilution of 1:2000

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112936(MYH7 Antibody) at dilution of 1:2000

    IP Result of anti-MYH7 (IP:Catalog No:112936, 5ug; Detection:Catalog No:112936 1:300) with mouse heart tissue lysate 4000ug.

    IP Result of anti-MYH7 (IP:Catalog No:112936, 5ug; Detection:Catalog No:112936 1:300) with mouse heart tissue lysate 4000ug.

    Immunohistochemistry of paraffin-embedded mouse heart tissue slide using Catalog No:112936(MYH7 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded mouse heart tissue slide using Catalog No:112936(MYH7 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded mouse heart tissue slide using Catalog No:112936(MYH7 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded mouse heart tissue slide using Catalog No:112936(MYH7 Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    Myosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs. (2523412, 14520662)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"