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Product Name
MUTYH antibody
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Description
MUTYH Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, COLO 320 cells, Jurkat cells, K-562 cells, mouse thymus tissue, Raji cells. Positive IP detected in HeLa cells. Positive IHC detected in human kidney tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 53-64 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hMYH antibody; MutY homolog antibody; mutY homolog (E. coli) antibody; MUTYH antibody; MYH antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MUTYH recombinant protein (Accession Number: NM_001293190). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112904(MUTYH antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:112904(MUTYH Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:112904(MUTYH Antibody) at dilution of 1:50
IP Result of anti-MUTYH (IP:Catalog No:112904, 4ug; Detection:Catalog No:112904 1:500) with HeLa cells lysate 3440ug.
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Background
MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.
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