Product Name

MUS81 antibody

Documents

Description

MUS81 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 52-72 kDa

Tested applications

ELISA, IHC, IF, WB

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

FLJ21012 antibody; FLJ44872 antibody; MUS81 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of MUS81 recombinant protein (Accession Number: NM_025128). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:10-1:100

IF: 1:20-1:200

Validations

Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112900(MUS81 antibody) at dilution of 1:10 (under 10x lens)

Immunofluorescent analysis of MCF-7 cells, using MUS81 antibody Catalog No:112900 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112900(MUS81 Antibody) at dilution of 1:1000

Background

The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).

MUS81 antibody

Product Information

Related Products / Services