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Product Name
MTHFD1 antibody
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Description
MTHFD1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IP detected in mouse kidney tissue. Positive WB detected in mouse kidney tissue, HEK-293 cells, human brain tissue. Observed molecular weight by Western-blot: 101 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
MTHFC antibody; MTHFD antibody; MTHFD1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MTHFD1 recombinant protein (Accession Number: NM_005956). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:112776(MTHFD1 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112776(MTHFD1 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-MTHFD1 (IP:Catalog No:112776, 5ug; Detection:Catalog No:112776 1:600) with mouse kidney tissue lysate 4000ug.
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Background
MTHFD1 represents methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
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References
- Piskounova E, Agathocleous M, Murphy MM. Oxidative stress inhibits distant metastasis by human melanoma cells. Nature. 527(7577):186-91. 2015.
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