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Product Name
MSI1 Polyclonal Antibody
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Description
Polyclonal antibody to MSI1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Rat
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Alternative names
MSI1 antibody; musashi RNA binding protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSI1 (NP_002433.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - MSI1 Polyclonal Antibody
Western blot analysis of extracts of A-549 cells, using MSI1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 30s.
Immunohistochemistry - MSI1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using MSI1 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - MSI1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using MSI1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"