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Product Name
MRPL28 antibody
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Description
MRPL28 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in A375 cells, HeLa cells, human kidney tissue, human testis tissue, mouse brain tissue, mouse liver tissue, mouse spleen tissue, mouse thymus tissue. Positive IHC detected in human kidney tissue, human testis tissue. Positive IF detected in HepG2 cells, Hela cells. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
L28mt antibody; MAAT1 antibody; Melanoma antigen p15 antibody; MRP L28 antibody; MRPL28 antibody; p15 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MRPL28 recombinant protein (Accession Number: XM_005255041). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:112821(MRPL28 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112821(MRPL28 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112821(MRPL28 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MRPL28 antibody Catalog No:112821 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MRPL28 (IP:Catalog No:112821, 4ug; Detection:Catalog No:112821 1:500) with mouse brain tissue lysate 4000ug.
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Background
MRPL28 represents mitochondrial ribosomal protein L28
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