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Product Name
MRPL11 antibody
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Description
MRPL11 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue. Positive FC detected in HepG2 cells. Positive IP detected in mouse liver tissue. Positive WB detected in HepG2 cells, K-562 cells, mouse liver tissue. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IHC, FC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 113 antibody; L11MT antibody; MRP L11 antibody; MRPL11 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MRPL11 recombinant protein (Accession Number: NM_016050). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112810(MRPL11 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:112810(MRPL11 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:112810(MRPL11 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MRPL11 (IP:Catalog No:112810, 4ug; Detection:Catalog No:112810 1:1000) with mouse liver tissue lysate 4000ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:112810(MRPL11 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug MRPL11 antibody (Catalog No:112810, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
MRPL11 is one of the protein components of mitochondrial ribosomes encoded by the nuclear genome, which is essential for oxidative phosphorylation.
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References
- Richter U, Lahtinen T, Marttinen P, Suomi F, Battersby BJ. Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. The Journal of cell biology. 211(2):373-89. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"