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Product Name
MPP8 antibody
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Description
MPP8 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, HEK-293 cells, HeLa cells, HepG2 cells, MCF7 cells, PC-3 cells. Observed molecular weight by Western-blot: 97-105 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ35237 antibody; HSMPP8 antibody; M phase phosphoprotein 8 antibody; MPHOSPH8 antibody; MPP8 antibody; TWA3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MPP8 recombinant protein (Accession Number: NM_017520). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112752(MPHOSPH8 antibody) at dilution of 1:1500
IP Result of anti-MPHOSPH8 (IP:Catalog No:112752, 4ug; Detection:Catalog No:112752 1:1000) with mouse brain tissue lysate 4000ug.
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Background
The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a) [PMID: 18726070].
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References
- Nishigaki M, Kawada Y, Misaki T. Mitotic phosphorylation of MPP8 by cyclin-dependent kinases regulates chromatin dissociation. Biochemical and biophysical research communications. 432(4):654-9. 2013.
- Murata K, Sato S, Haruta M. Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis. Biochemical and biophysical research communications. 458(3):470-5. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"