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Product Name
MPG Polyclonal Antibody
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Description
Polyclonal antibody to MPG
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MPG antibody; AAG antibody; ADPG antibody; APNG antibody; CRA36.1 antibody; MDG antibody; Mid1 antibody; PIG11 antibody; PIG16 antibody; anpg antibody; N-methylpurine DNA glycosylase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - MPG Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MPG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 40s.
Immunofluorescence - MPG Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using MPG antibody . Blue: DAPI for nuclear staining.
Immunofluorescence - MPG Polyclonal Antibody
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MPG antibody . Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence - MPG Polyclonal Antibody
Immunofluorescence analysis of A549 cells using MPG antibody .
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Background
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"