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MPG antibody

Cat.#: 112742

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Special Price 239.0 USD

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Product Information

  • Product Name
    MPG antibody
  • Documents
  • Description
    MPG Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IHC detected in human prostate cancer. Observed molecular weight by Western-blot: 33kd
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    human, mouse, rat; other species not tested.
  • Alternative names
    MPG antibody; PIG11 antibody; N methylpurine DNA glycosylase antibody; MDG antibody; 3 alkyladenine DNA glycosylase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MPG recombinant protein (Accession Number: NM_001015052). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112742(MPG antibody) at dilution of 1:100

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112742(MPG antibody) at dilution of 1:100

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112742(MPG antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112742(MPG antibody) at dilution of 1:50 (under 10x lens)

  • Background
    N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726].
  • References
    • Yamamoto M, Yamamoto R, Takenaka S, Matsuyama S, Kubo K. Abundance of BER-related proteins depends on cell proliferation status and the presence of DNA polymerase β. Journal of radiation research. 56(4):607-14. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"