Product Name

MOV10 antibody

Documents

Description

MOV10 Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells, human liver tissue, mouse liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 110-115 kDa

Tested applications

ELISA, WB, IHC, IP, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DKFZp667O1423 antibody; FLJ32791 antibody; fSAP113 antibody; gb110 antibody; KIAA1631 antibody; MOV10 antibody; Putative helicase MOV 10 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of MOV10 recombinant protein (Accession Number: NM_020963). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112738(MOV10 antibody) at dilution of 1:800

IP Result of anti-MOV10 (IP:Catalog No:112738, 3ug; Detection:Catalog No:112738 1:800) with HepG2 cells lysate 1000ug.

Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112738(MOV10 antibody) at dilution of 1:50 (under 25x lens)

Immunofluorescent analysis of HepG2 cells using Catalog No:112738(MOV10 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Background

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

References

Haussecker D, Cao D, Huang Y, Parameswaran P, Fire AZ, Kay MA. Capped small RNAs and MOV10 in human hepatitis delta virus replication. Nature structural & molecular biology. 15(7):714-21. 2008.
Ma YX, Li D, Fu LJ. The role of Moloney leukemia virus 10 in hepatitis B virus expression in hepatoma cells. Virus research. 197:85-91. 2015.
Johnson ME, Grassetti AV, Taroni JN. Stress granules and RNA processing bodies are novel autoantibody targets in systemic sclerosis. Arthritis research & therapy. 18(1):27. 2016.
Han Y, Wang X, Dang Y, Zheng YH. APOBEC3G and APOBEC3F require an endogenous cofactor to block HIV-1 replication. PLoS pathogens. 4(7):e1000095. 2008.
Cao D, Haussecker D, Huang Y, Kay MA. Combined proteomic-RNAi screen for host factors involved in human hepatitis delta virus replication. RNA (New York, N.Y.). 15(11):1971-9. 2009.
Furtak V, Mulky A, Rawlings SA. Perturbation of the P-body component Mov10 inhibits HIV-1 infectivity. PloS one. 5(2):e9081. 2010.
Banerjee S, Neveu P, Kosik KS. A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation. Neuron. 64(6):871-84. 2009.
Wang X, Han Y, Dang Y. Moloney leukemia virus 10 (MOV10) protein inhibits retrovirus replication. The Journal of biological chemistry. 285(19):14346-55. 2010.

MOV10 antibody

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