Mouse SRC/Proto-oncogene c-Src (His & GST Tag) recombinant protein

Mouse SRC/Proto-oncogene c-Src (His & GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

SRC1; AW259666; C-SRC; PP60C-SRC; RP5-823N20.1; SRC; P60-SRC; ASV; RP23-169M4.1

Q80XU2

Baculovirus-Insect Cells

A DNA sequence encoding the mouse SRC (NP_001020566.111) (Met 1-Leu 535) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Mouse

The recombinant mouse SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. The specific activity was determined to be 166 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.
2. Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse SRC at 2 μg/ml (100 μl/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/ml.