Mouse Nogo Receptor (His & Fc Tag) recombinant protein

Mouse Nogo Receptor (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Reticulon 4 receptor (RTN4R), also known as Nogo-66 Receptor (NgR), is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the Nogo recptor family including three members. Mouse RTN4R cDNA contains 10 LRP (Leucine-rich) repeats. RTN4R is expressed predominantly in neurons and their axons in the central nervous systems (CNS). As a receptor for myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMG), RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult CNS. It has been shown that RTN4R performs its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a TNFRSF member also known for modulating the activities of the Trk family and for inducing apoptosis in neurons and oligodendrocytes. RTN4R may be proposed as a potential drug target for treatment of various neurological conditions such as spinal cord injury, CNS lesions, peripheral nerve injury, stroke and Alzheimer's disease (AD). Additionally, RTN4R may play a role in regulating the function of the gap junctions.

Reticulon-4 receptor

NGR1; NOGOR; UNQ330/PRO526; NGR

Q99PI8

Rtn4r; Nogor

Human Cells

A DNA sequence encoding the the mature form of mouse RTN4R (NP_075358.1) (Met 1-Ser 447) was fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse RTN4R/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 669 amino acids and has a predicted molecular mass of 73.8 kDa. Due to glycosylation, rmRTN4R/Fc monomer migrates as an approximately 95-100 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized recombinant Mouse RTN4R Fc Chimera at 2 μg/ml (100 μl/well) can bind biotinylated human RTN4 (GST Tag) with a linear range of 0.16-2.5 μg/ml.