Mouse M-CSF/CSF-1 (His Tag) recombinant protein

Mouse M-CSF/CSF-1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

Macrophage colony-stimulating factor 1

MGC31930; MCSF; C87615; CSF1; M-CSF; RP11-195M16.2; CSF-1; CSFM; OP

P07141

Csf1; Csfm

Human Cells

A DNA sequence encoding the mouse CSF1 (P07141-1) (Met1-Glu262) was expressed with a C-terminal polyhistidine tag.

Mouse

The recombinant mouse CSF1 comprises 241 amino acids and has a predicted molecular mass of 27.4 kDa. The apparent molecular mass of the protein is approximately 40-55 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Immobilized mouse CSF1-His at 10 μg/ml (100 μl/well) can bind mouse CSF1R-Fch (Cat:504717), The EC₅₀ of mouse CSF1R-Fch (Cat:504717) is 0.04-0.1 μg/ml.
2. Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is typically 2-10 ng/ml.