Mouse Fetuin-A (His Tag) recombinant protein

Mouse Fetuin-A (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.

Fibroblast growth factor receptor 4

AHSG; A2HS; HSGA; AHSG]; FETUA; FETUIN-A; AHS

Q03142

Fgfr4; Fgfr-4; Mpk-11

Human Cells

A DNA sequence encoding the mouse AHSG (NP_032037.2) precursor (Met1-Ile 345) was expressed with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse AHSG consists of 338 amino acids and has a predicted molecular mass of 36.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm AHSG is approximately 50 kDa due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized human FCN1 at 10 μg/mL can bind biotinylated recombinant mouse Fetuin-A with a linear range of 16-2000 ng/ml.
2. Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, AnaSpec, Catalog # 27114. The IC50 value is <200 nM.