Rat FGFR4/CD334 (Fc Tag) recombinant protein

Rat FGFR4/CD334 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4 (By similarity).

Fibroblast growth factor receptor 4

TKF; JTK2; FGFR-4; CD334; FGFR4; MGC20292

Q498D6

Fgfr4

Human Cells

A DNA sequence encoding the rat FGFR4 (Q498D6) extracellular domain (Met 1-Asp 367) was fused with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat FGFR4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 592 amino acids and predicts a molecular mass of 66 kDa. The apparent molecular mass of the rat FGFR4/Fc monomer is approximately 110 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human FGF18 (Cat: 500546) at 10 μg/mL (100 μl/well) can bind  Rat FGFR4 (Cat:500770), The EC₅₀ of Rat FGFR4 (Cat:500770) is 1.17 μg/mL.
3. Immobilized mouse FGF18 (Cat:501491) at 10 μg/mL (100 μl/well) can bind  Rat FGFR4 (Cat:500770), The EC₅₀ of Rat FGFR4 (Cat:500770) is 0.44 μg/mL.
4. Immobilized human bFGF at 10 μg/mL (100 μl/well) can bind  Rat FGFR4 (Cat:500770), The EC₅₀ of Rat FGFR4 (Cat:500770) is 0.163 μg/mL.