Mouse Epiregulin/EREG (Fc Tag) recombinant protein

Mouse Epiregulin/EREG (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Epiregulin (EREG) is a member of the epidermal growth factor family. Epiregulin (EREG) can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. Epiregulin (EREG) exhibit bifunctional regulatory properties: it inhibit the growth of several epithelial tumor cells and stimulated the growth of fibroblasts and various other types of cells. Epiregulin (EREG) bound to the EGF receptors of epidermoid carcinoma A431 cells much more weakly than did EGF, but was nevertheless much more potent than EGF as a mitogen for rat primary hepatocytes and Balb/c 3T3 A31 fibroblasts. These findings suggest that epiregulin (EREG) plays important roles in regulating the growth of epithelial cells and fibroblasts by binding to receptors for EGF-related ligands. Epiregulin (EREG) is the broadest specificity EGF-like ligand so far characterized: not only does it stimulate homodimers of both ErbB-1 and ErbB-4, it also activates all possible heterodimeric ErbB complexes.

Proepiregulin

MGC36144; EREG; EPR; ER; PROEPIREGULIN; EPIREGULIN

Q61521

Ereg

Human Cells

A DNA sequence encoding the mature form of mouse EREG (Q61521) (Val 56-Leu 101) was fused with the Fc region of human IgG1 at the N-terminus.

Mouse

The recombinant mouse EREG/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 306 amino acids and has a predicted molecular mass of 33.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEREG/Fc monomer is approximately 37 kDa.

> 95 % as determined by SDS-PAGE

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
The ED₅₀ for this effect is typically 0.1-0.5 μg/mL.