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Product Name
MOK antibody
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Description
MOK Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells. Positive FC detected in HeLa cells. Positive IHC detected in human hepat℃irrhosis tissue, human heart tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 42 kDa,85 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human; other species not tested.
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Alternative names
MOK antibody; MOK protein kinase antibody; RAGE antibody; RAGE 1 antibody; RAGE1 antibody; renal tumor antigen antibody; Renal tumor antigen 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MOK recombinant protein (Accession Number: BC053536). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112732(RAGE Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:112732(RAGE Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:112732(RAGE Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:112732(RAGE Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug RAGE antibody (Catalog No:112732, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
RAGE represents renal tumor antigen
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