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Product Name
MMS19 antibody
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Description
MMS19 Mouse Monoclonal antibody. Positive WB detected in Hepg2 cells, human brain tissue, human kidney tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human cervical cancer tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 103 kDa,38 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
FLJ34167 antibody; FLJ95146 antibody; hMMS19 antibody; MET18 antibody; MET18 homolog antibody; MMS19 antibody; MMS19 like protein antibody; MMS19L antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of MMS19 recombinant protein (Accession Number: BC006575). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107428(MMS19 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:107428(MMS19 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using MMS19 antibody Catalog No: at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
IP Result of anti-MMS19 (IP:Catalog No:107428, 4ug; Detection:Catalog No:107428 1:2000) with mouse brain tissue lysate 4000ug.
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Background
MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.
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References
- Stehling O, Vashisht AA, Mascarenhas J. MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity. Science (New York, N.Y.). 337(6091):195-9. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"