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Product Name
MMP28 antibody
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Description
MMP28 Rabbit Polyclonal antibody. Positive IF detected in A549 cells. Positive IHC detected in human kidney tissue, human ovary tissue, human skin cancer tissue, human testis tissue. Positive WB detected in A549 cells, human colon tissue, mouse heart tissue. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EPILYSIN antibody; matrix metallopeptidase 28 antibody; Matrix metalloproteinase 28 antibody; MM28 antibody; MMP 28 antibody; MMP28 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MMP28 recombinant protein (Accession Number: NM_032950). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:112705(MMP28 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112705(MMP28 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112705(MMP28 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed A549 cells using Catalog No:112705(MMP28 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.
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References
- Zhang M, Liu Y, Feng H. CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2. The American journal of pathology. 182(2):565-76. 2013.
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