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Product Name
MMP19 antibody
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Description
MMP19 Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive IF detected in SH-SY5Y cells. Positive IP detected in human placenta tissue. Positive WB detected in SH-SY5Y cells, human placenta tissue. Observed molecular weight by Western-blot: 57-60 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
matrix metallopeptidase 19 antibody; Matrix metalloproteinase 18 antibody; Matrix metalloproteinase 19 antibody; Matrix metalloproteinase RASI antibody; MMP 18 antibody; MMP 19 antibody; MMP18 antibody; MMP19 antibody; RASI antibody; RASI 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MMP19 recombinant protein (Accession Number: NM_002429). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:112699(MMP19 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:112699(MMP19 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MMP19 (IP:Catalog No:112699, 4ug; Detection:Catalog No:112699 1:500) with human placenta tissue lysate 1520ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed SH-SY5Y cells using Catalog No:112699(MMP19 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MMP19(matrix metalloproteinase-19) has the capacity to degrade several basement membrane proteins such as type IV collagen, laminin, tenascin C, and nidogen. It is expressed by microvascular endothelial cells in vitro as well as by capillaries in the inflamed synovial membrane, whereas its expression is absent in quiescent macrovascular endothelial cells of large arteries and veins . And it is also detected in capillaries of normal mammary tissue, benign mammary carcinomas and in skin capillaries(PMID:15241558).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"