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Product Name
MMP16 Polyclonal Antibody
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Description
Polyclonal antibody to MMP16
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MMP16 antibody; C8orf57 antibody; MMP-X2 antibody; MT-MMP2 antibody; MT-MMP3 antibody; MT3-MMP antibody; matrix metallopeptidase 16 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-565 of human MMP16 (NP_005932.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - MMP16 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MMP16 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - MMP16 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using MMP16 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MMP16 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP16 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MMP16 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast using MMP16 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MMP16 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using MMP16 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - MMP16 Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using MMP16 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"