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MME,CD10 antibody

Cat.#: 112694

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Product Information

  • Product Name
    MME,CD10 antibody
  • Documents
  • Description
    MME,CD10 Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, human kidney tissue, rat kidney tissue. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 90-110 kDa
  • Tested applications
    ELISA, WB, IF, FC
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    Atriopeptidase antibody; CALLA antibody; CD10 antibody; DKFZp686O16152 antibody; Enkephalinase antibody; EPN antibody; membrane metallo endopeptidase antibody; MME antibody; NEP antibody; Neprilysin antibody; Neutral endopeptidase antibody; Neutral endopeptidase 24.11 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MME,CD10 recombinant protein (Accession Number: NM_001354643). Purification method: Antigen Affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:20-1:200

  • Validations

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:112694(MME,CD10 antibody) at dilution of 1:1000

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:112694(MME,CD10 antibody) at dilution of 1:1000

    Immunofluorescent analysis of HepG2 cells, using MME antibody Catalog No:112694 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using MME antibody Catalog No:112694 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 HepG2 cells were stained with .2ug MME,CD10 antibody (Catalog No:112694, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with .2ug MME,CD10 antibody (Catalog No:112694, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"