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Product Name
MLK3 antibody
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Description
MLK3 Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, Jurkat cells. Positive IF detected in Hela cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 60kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MAP3K11 antibody; Mixed lineage kinase 3 antibody; MLK 3 antibody; MLK3 antibody; PTK1 antibody; SPRK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MLK3 recombinant protein (Accession Number: NM_002419). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112679(MAP3K11 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112679(MAP3K11 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using MAP3K11 antibody Catalog No:112679 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
MLK3(Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439).
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