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Product Name
MLANA antibody
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Description
MLANA Rabbit Polyclonal antibody. Positive WB detected in Transfected HEK-293 cells. Positive IHC detected in human malignant melanoma tissue, human skin tissue.
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
Antigen LB39 AA antibody; Antigen SK29 AA antibody; MART 1 antibody; MART1 antibody; melan A antibody; MLANA antibody; Protein Melan A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MLANA recombinant protein (Accession Number: NM_005511). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112672(MLANA Antibody) at dilution of 1:700
Immunohistochemistry of paraffin-embedded human malignant melanoma tissue slide using Catalog No:112672(MLANA Antibody) at dilution of 1:50 (under 40x lens)
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Background
Protein melan-A also known as MLANA or MART-1 is a protein that in humans is encoded by the MLANA gene. A fragment of the protein, usually consisting of the nine amino acids 27 to 35, is bound by MHC class I complexes which present it to T cells of the immune system. These complexes can be found on the surface of melanoma cells. Decameric peptides (26-35) are being investigated as cancer vaccines. Its expression is regulated by the Microphthalmia-associated transcription factor. (refer to Wikipedia)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"