Product Name

MGME1 antibody

Documents

Description

MGME1 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human liver cancer tissue. Positive WB detected in Jurkat cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, Y79 cells. Observed molecular weight by Western-blot: 36-39 kDa

Tested applications

ELISA, WB, IHC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

bA504H3.4 antibody; C20orf72 antibody; DDK1 antibody; FLJ14597 antibody; MGME1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of MGME1 recombinant protein (Accession Number: XM_017028127). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

Validations

Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112602(MGME1 antibody) at dilution of 1:1000

Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:112602(MGME1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:112602(MGME1 Antibody) at dilution of 1:50 (under 40x lens)

Background

MGME1, also named as C20orf72, is a single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. It has preference for 5'-3' exonuclease activity. MGME1 cleaves single-stranded DNA (ssDNA) and several DNA or DNA-RNA chimeric oligonucleotides that showed free nucleic acid ends. It probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. This antibody detects the 35-40 kDa protein.

MGME1 antibody

Product Information

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