-
Product Name
MFF Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to MFF
-
Tested applications
WB
-
Species reactivity
Human
-
Alternative names
MFF antibody; C2orf33 antibody; EMPF2 antibody; GL004 antibody; mitochondrial fission factor antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 15-170 of human MFF (NP_064579.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:200 - 1:500
-
Validations
Western blot - MFF Polyclonal Antibody
Western blot analysis of extracts of LO2 cells, using MFF antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
-
Background
Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"