Product Name

MFF antibody

Documents

Description

MFF Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, Hela cells. Positive IHC detected in human brain tissue, human heart tissue, human stomach tissue. Positive WB detected in human brain tissue, HeLa cells, mouse brain tissue, rat brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 26-29, 35-38 kDa

Tested applications

ELISA, WB, IHC, IP, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

C2orf33 antibody; MFF antibody; mitOChondrial fission factor antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of MFF recombinant protein (Accession Number: XM_006712639). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

IP Result of anti-MFF (IP:Catalog No:112527, 3ug; Detection:Catalog No:112527 1:1000) with mouse brain tissue lysate 8000ug.

Immunohistochemical of paraffin-embedded human brain using Catalog No:112527(MFF antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human brain using Catalog No:112527(MFF antibody) at dilution of 1:100 (under 40x lens)

human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112527(MFF antibody) at dilution of 1:1000

Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:112527(MFF Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

References

Friedman JR, Lackner LL, West M, DiBenedetto JR, Nunnari J, Voeltz GK. ER tubules mark sites of mitochondrial division. Science (New York, N.Y.). 334(6054):358-62. 2011.
Li S, Xu S, Roelofs BA. Transient assembly of F-actin on the outer mitochondrial membrane contributes to mitochondrial fission. The Journal of cell biology. 208(1):109-23. 2015.
Mason TA, Goldenring JR, Kolobova E. AKAP350C targets to mitochondria via a novel amphipathic alpha helical domain. Cellular logistics. 4(3):e943597. 2015.
Ducommun S, Deak M, Sumpton D. Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: identification of mitochondrial fission factor as a new AMPK substrate. Cellular signalling. 27(5):978-88. 2015.
Gao J, Qin S, Jiang C. Parkin-induced ubiquitination of Mff promotes its association with p62/SQSTM1 during mitochondrial depolarization. Acta biochimica et biophysica Sinica. 47(7):522-9. 2015.
Qi X, Qvit N, Su YC, Mochly-Rosen D. A novel Drp1 inhibitor diminishes aberrant mitochondrial fission and neurotoxicity. Journal of cell science. 126(Pt 3):789-802. 2013.
Ban-Ishihara R, Ishihara T, Sasaki N, Mihara K, Ishihara N. Dynamics of nucleoid structure regulated by mitochondrial fission contributes to cristae reformation and release of cytochrome c. Proceedings of the National Academy of Sciences of the United States of America. 110(29):11863-8. 2013.
Wang DB, Uo T, Kinoshita C. Bax interacting factor-1 promotes survival and mitochondrial elongation in neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34(7):2674-83. 2014.

MFF antibody

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