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Product Name
METAP2 antibody
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Description
METAP2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue. Positive WB detected in HepG2 cells, MCF7 cells. Positive IP detected in MCF-7 cells. Observed molecular weight by Western-blot: 67 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MAP 2 antibody; MAP2 antibody; MetAP 2 antibody; METAP2 antibody; Methionine aminopeptidase 2 antibody; methionyl aminopeptidase 2 antibody; MNPEP antibody; p67 antibody; p67eIF2 antibody; Peptidase M 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of METAP2 recombinant protein (Accession Number: NM_006838). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112514(METAP2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human testis using Catalog No:112514(METAP2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:112514(METAP2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-METAP2 (IP:Catalog No:112514, 4ug; Detection:Catalog No:112514 1:300) with MCF-7 cells lysate 3200ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:112514(METAP2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
METAP2 represents methionyl aminopeptidase 2
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