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Product Name
Mouse TIMP-1/TIMP1 recombinant protein
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Description
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
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Protein name
Metalloproteinase inhibitor 1
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Protein short names
EPO; CLGI; RP23-132C19.3; TIMP; HCI; FLJ90373; EPA; TIMP-1; TPA-S1; TIMP1; RP1-230G1.3; MGC7143
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Uniprot ID
P12032
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Gene Name
Timp1; Timp; Timp-1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse TIMP1 (NP_035723.2) (Met 1-Arg 205) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse TIMP1 consists of 181 amino acids and has a calculated molecular mass of 21 kDa. It migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2(R&D Systems, Catalog # ES001). The IC50 value is < 6 nM.
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Validations
Mouse TIMP1 / TIMP / CLGI Protein SDS-PAGE
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