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Anti-TIMP1 antibody

Cat.#: 175096

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-TIMP1 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to TIMP1
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    EPA antibody; EPO antibody; HCI antibody; CLGI antibody; TIMP antibody; TIMP-1 antibody
  • Isotype
    Mouse IgG
  • Preparation
    This antigen of this antibody was recombinant human timp-1
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    IHC-P:1:400-800

    IF:1:200-800

    ICC/IF:1:100-500

  • Validations

    Fig1: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

    Fig1: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

    Fig2: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

    Fig2: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

    Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

    Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

    Fig4: Sample:; Recombinant human TIMP-1 Protein Lysate at 40 ug; Primary: Anti-TIMP-1 at 1/50000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 21 kD; Observed band size: 19 kD

    Fig4: Sample:; Recombinant human TIMP-1 Protein Lysate at 40 ug; Primary: Anti-TIMP-1 at 1/50000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 21 kD; Observed band size: 19 kD

    Fig5: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

    Fig5: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

    Fig6: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

    Fig6: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

    Fig7: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30mi

    Fig7: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30mi

    Fig8: Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37

    Fig8: Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37

  • Background
    P01033

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