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Product Name
MEPE antibody
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Description
MEPE Rabbit Polyclonal antibody. Positive IHC detected in human lung tissue. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, MCF7 cells. Observed molecular weight by Western-blot: 98 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
MEPE antibody; OF45 antibody; Osteoblast/osteOCyte factor 45 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MEPE recombinant protein (Accession Number: NM_020203). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112659(MEPE antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human lung slide using Catalog No:112659(MEPE Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lung slide using Catalog No:112659(MEPE Antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-MEPE (IP:Catalog No:112659, 4ug; Detection:Catalog No:112659 1:1000) with HeLa cells lysate 4000ug.
Immunofluorescent analysis of HepG2 cells, using MEPE antibody Catalog No:112659 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases. (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a full-length human MEPE protein detects a major band of 98 kDa.
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