Product Name

Melanophilin antibody

Documents

Description

Melanophilin Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human skin cancer tissue, human kidney tissue. Positive WB detected in PC-3 cells, HEK-293 cells. Observed molecular weight by Western-blot: 66kD,63kD

Tested applications

ELISA, WB, IF, IHC

Species reactivity

Human; other species not tested.

Alternative names

Exophilin 3 antibody; melanophilin antibody; MLPH antibody; SLAC2 A antibody; SLAC2A antibody; Synaptotagmin like protein 2a antibody

Isotype

Mouse IgG1

Preparation

This antibody was obtained by immunization of Melanophilin recombinant protein (Accession Number: NM_024101). Purification method: Protein G purified.

Clonality

Monoclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:107422(MLPH antibody) at dilution of 1:1000

Immunohistochemistry of paraffin-embedded human skin cancer slide using Catalog No:107422(MLPH Antibody) at dilution of 1:50

Immunofluorescent analysis of HepG2 cells, using MLPH antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Background

Melanophilin (MLPH) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

Melanophilin antibody

Product Information

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