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Product Name
ME2 antibody
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Description
ME2 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in K-562 cells, HeLa cells, HL-60 cells, Jurkat cells. Observed molecular weight by Western-blot: 54 kDa, 65 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Malic enzyme 2 antibody; ME2 antibody; NAD ME antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ME2 recombinant protein (Accession Number: NM_001168335). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:112575(ME2 Antibody) at dilution of 1:600
IP Result of anti-ME2 (IP:Catalog No:112575, 4ug; Detection:Catalog No:112575 1:500) with HeLa cells lysate 3200ug.
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Background
ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter γ-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.
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References
- Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.
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