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Product Name
MCM7 antibody
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Description
MCM7 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, A431 cells, HeLa cells, MCF7 cells. Positive IP detected in NIH/3T3 cells. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human lung cancer tissue, human lymphoma tissue. Positive FC detected in Raji cells. Observed molecular weight by Western-blot: 81 kDa
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Tested applications
ELISA, WB, IP, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDABP0042 antibody; CDC47 antibody; CDC47 homolog antibody; MCM2 antibody; MCM7 antibody; P1.1 MCM3 antibody; P1CDC47 antibody; P85MCM antibody; PNAS 146 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MCM7 recombinant protein (Accession Number: NM_005916). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112557(MCM7 Antibody) at dilution of 1:3000
IP Result of anti-MCM7 (IP:Catalog No:112557, 4ug; Detection:Catalog No:112557 1:2000) with NIH/3T3 cells lysate 1600ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:112557(MCM7 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:112557(MCM7 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 Raji cells were stained with 0.2ug MCM7 antibody (Catalog No:112557, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].
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References
- Du R, Long J, Yao J. Subcellular quantitative proteomics reveals multiple pathway cross-talk that coordinates specific signaling and transcriptional regulation for the early host response to LPS. Journal of proteome research. 9(4):1805-21. 2010.
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