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Product Name
MCM6 antibody
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Description
MCM6 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue, HeLa cells, Jurkat cells, mouse ovary tissue, mouse spleen tissue, mouse thymus tissue. Positive IP detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human lung cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 105 kDa
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Tested applications
ELISA, WB, IP, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MCG40308 antibody; MCM6 antibody; Mis5 antibody; P105MCM antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MCM6 recombinant protein (Accession Number: NM_005915). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:112556(MCM6 antibody) at dilution of 1:500
IP Result of anti-MCM6 (IP:Catalog No:112556, 3ug; Detection:Catalog No:112556 1:1000) with HeLa cells lysate 3800ug.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:112556(MCM6 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:112556(MCM6 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027].
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References
- Liu YZ, Jiang YY, Wang BS. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer cytopathology. 122(11):833-41. 2014.
- Yuan Q, Han J, Cong W. Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity. International journal of nanomedicine. 9:4829-46. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"