Product Name

MCL1 antibody

Documents

Description

MCL1 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue, human cervical cancer tissue, human lung cancer tissue, human pancreas tissue. Positive IP detected in Raji cells. Positive WB detected in Raji cells, A431 cells, HeLa cells, HL-60 cells, human brain tissue, human liver tissue, Jurkat cells, K-562 cells, mouse spleen tissue. Observed molecular weight by Western-blot: 40 kDa

Tested applications

ELISA, IHC, WB, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

EAT antibody; Mcl 1 antibody; Mcl 1l antibody; Mcl 1s Delta TM antibody; MCL1 antibody; mcl1/EAT antibody; MCL1L antibody; MCL1S antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of MCL1 recombinant protein (Accession Number: NM_021960). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

Validations

Immunohistochemistry of paraffin-embedded human tonsillitis slide using Catalog No:112549(MCL1 Antibody) at dilution of 1:50

Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:112549(MCL1 antibody) at dilution of 1:1000

IP Result of anti-MCL1 (IP:Catalog No:112549, 3ug; Detection:Catalog No:112549 1:1000) with Raji cells lysate 800ug.

Background

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target.

References

Jiang Y, Wang W, Wang J. Functional regulatory variants of MCL1 contribute to enhanced promoter activity and reduced risk of lung cancer in nonsmokers: implications for context-dependent phenotype of an antiapoptotic and antiproliferative gene in solid tumor. Cancer. 118(8):2085-95. 2012.
Xu H, Jiang B, Meng L. N-α-acetyltransferase 10 protein inhibits apoptosis through RelA/p65-regulated MCL1 expression. Carcinogenesis. 33(6):1193-202. 2012.
Gong K, Chen C, Zhan Y, Chen Y, Huang Z, Li W. Autophagy-related gene 7 (ATG7) and reactive oxygen species/extracellular signal-regulated kinase regulate tetrandrine-induced autophagy in human hepatocellular carcinoma. The Journal of biological chemistry. 287(42):35576-88. 2012.
Yu G, Jiang L, Xu Y. Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli. PloS one. 7(11):e48146. 2012.
Wu KN, Zhao YM, He Y. Rapamycin interacts synergistically with idarubicin to induce T-leukemia cell apoptosis in vitro and in a mesenchymal stem cell simulated drug-resistant microenvironment via Akt/mammalian target of rapamycin and extracellular signal-related kinase signaling pathways. Leukemia & lymphoma. 55(3):668-76. 2014.
Wu X, Deng G, Hao X. A caspase-dependent pathway is involved in Wnt/β-catenin signaling promoted apoptosis in Bacillus Calmette-Guerin infected RAW264.7 macrophages. International journal of molecular sciences. 15(3):5045-62. 2014.
Wang S, Jiang Y, Liu J. Revisiting the role of MCL1 in tumorigenesis of solid cancer: gene expression correlates with antiproliferative phenotype in breast cancer cells and its functional regulatory variants are associated with reduced cancer susceptibility. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 35(8):8289-99. 2014.
Zhang Q, Zhang Y, Zhang P. Hexokinase II inhibitor, 3-BrPA induced autophagy by stimulating ROS formation in human breast cancer cells. Genes & cancer. 5(3-4):100-12. 2014.

MCL1 antibody

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