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Product Name
MBD2 Polyclonal Antibody
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Description
Polyclonal antibody to MBD2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MBD2 antibody; DMTase antibody; NY-CO-41 antibody; methyl-CpG-binding domain protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 202-411 of human MBD2 (NP_003918.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - MBD2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MBD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - MBD2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using MBD2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MBD2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using MBD2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MBD2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human uterine cancer using MBD2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MBD2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using MBD2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MBD2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using MBD2 antibody at dilution of 1:100 (40x lens).
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Background
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"