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Product Name
MAT1A antibody
- Documents
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Description
MAT1A Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, arabidopsis whole plant, HepG2 cells. Positive IHC detected in human lung tissue. Observed molecular weight by Western-blot: 44-55kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AdoMet synthase 1 antibody; AMS1 antibody; MAT antibody; MAT 1 antibody; MAT I/III antibody; MAT1A antibody; MATA1 antibody; SAMS antibody; SAMS1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MAT1A recombinant protein (Accession Number: XM_005269842). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
WB result of MAT1A antibody (Catalog No:112492, 1:5000) with plant leaf tissue samples.
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:112492(MAT1A antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung using Catalog No:112492(MAT1A antibody) at dilution of 1:200 (under 40x lens)
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Background
Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and may cross-react with MAT2A. Western blot analysis using this antibody detected a single band around 44-50kD in liver.
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References
- Li J, Ramani K, Sun Z. Forced expression of methionine adenosyltransferase 1A in human hepatoma cells suppresses in vivo tumorigenicity in mice. The American journal of pathology. 176(5):2456-66. 2010.
- Thomas A, Stevens AP, Klein MS. Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet. Proteomics. 12(9):1437-51. 2012.
- Vitvitsky V, Martinov M, Ataullakhanov F, Miller RA, Banerjee R. Sulfur-based redox alterations in long-lived Snell dwarf mice. Mechanisms of ageing and development. 134(7-8):321-30. 2013.
- Chen M, Zhou K, Chen X. Metabolomic analysis reveals metabolic changes caused by bisphenol A in rats. Toxicological sciences : an official journal of the Society of Toxicology. 138(2):256-67. 2014.
- Brown-Borg HM, Rakoczy S, Wonderlich JA, Armstrong V, Rojanathammanee L. Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice. Longevity & healthspan. 3(1):10. 2014.
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