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Product Name
MARK1 antibody
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Description
MARK1 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, HeLa cells, mouse heart tissue, mouse kidney tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 85 kDa,89 kDa,72 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA1477 antibody; MARK antibody; MARK1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MARK1 recombinant protein (Accession Number: NM_018650). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112478(MARK1 antibody) at dilution of 1:500
IP Result of anti-MARK1 (IP:Catalog No:112478, 4ug; Detection:Catalog No:112478 1:1000) with mouse brain tissue lysate 3440ug.
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Background
MARK1 represents MAP/microtubule affinity-regulating kinase 1
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References
- Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ. An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Molecular cell. 55(3):436-50. 2014.
- Bernard LP, Zhang H. MARK/Par1 Kinase Is Activated Downstream of NMDA Receptors through a PKA-Dependent Mechanism. PloS one. 10(5):e0124816. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"