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Product Name
MAPKAPK5 antibody
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Description
MAPKAPK5 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, Jurkat cells. Positive IP detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MAPKAP kinase 5 antibody; MAPKAPK 5 antibody; MAPKAPK5 antibody; PRAK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MAPKAPK5 recombinant protein (Accession Number: NM_003668). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112441(MAPKAPK5 antibody) at dilution of 1:1000
IP Result of anti-MAPKAPK5 (IP:Catalog No:112441, 4ug; Detection:Catalog No:112441 1:500) with HeLa cells lysate 2800ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:112441(MAPKAPK5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MAPKAPK5(MAP kinase-activated protein kinase 5) is also named as PRAK and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK5 contains the conserved protein kinase domains I through XI, which are characteristic of all protein kinases. It is a major stress-activated kinase that can phosphorylate small heat shock protein, suggesting a potential role for PRAK in mediating stress-induced HSP27 phosphorylation in vivo(PMID:9628874). It has 2 isoforms produced by alternative splicing.
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References
- Dwyer SF, Gelman IH. Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility. Journal of cancer biology & research. 2(1):. 2014.
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