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Product Name
MAD1L1 Polyclonal Antibody
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Description
Polyclonal antibody to MAD1L1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
MAD1L1 antibody; MAD1 antibody; PIG9 antibody; TP53I9 antibody; TXBP181 antibody; MAD1 mitotic arrest deficient like 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1L1 (NP_001013859.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - MAD1L1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MAD1L1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Immunohistochemistry - MAD1L1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using MAD1L1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MAD1L1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using MAD1L1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - MAD1L1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using MAD1L1 antibody .
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Background
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
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