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Product Name
M6PR Polyclonal Antibody
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Description
Polyclonal antibody to M6PR
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
M6PR antibody; CD-M6PR antibody; CD-MPR antibody; MPR 46 antibody; MPR-46 antibody; MPR46 antibody; SMPR antibody; cation-dependent mannose-6-phosphate receptor antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-185 of human M6PR (NP_002346.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - M6PR Polyclonal Antibody
Western blot analysis of extracts of mouse kidney, using M6PR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.
Immunohistochemistry - M6PR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using M6PR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - M6PR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using M6PR antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - M6PR Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using M6PR antibody at dilution of 1:100 (40x lens).
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Background
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"