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Product Name
LRPPRC antibody
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Description
LRPPRC Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human kidney tissue. Positive IF detected in HepG2 cells, HEK-293 cells, Hela cells. Positive IP detected in HeLa cells. Positive WB detected in HepG2 cells, HeLa cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, rat kidney tissue, rat liver tissue. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
130 kDa leucine rich protein antibody; CLONE 23970 antibody; GP130 antibody; LRP 130 antibody; LRP130 antibody; LRPPRC antibody; LSFC antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LRPPRC recombinant protein (Accession Number: NM_133259). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112325(LRPPRC antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:112325(LRPPRC antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:112325(LRPPRC antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using LRPPRC antibody Catalog No:112325 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-LRPPRC (IP:Catalog No:112325, 5ug; Detection:Catalog No:112325 1:1000) with HeLa cells lysate 1200ug.
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Background
LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.
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References
- Chen L, Lai Y, Zhu X. The role of specific PP2A complexes in the dephosphorylation of γH2AX. Journal of cell science. 128(2):421. 2015.
- Ma Y, Yao N, Liu G. Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells. The EMBO journal. 34(3):361-78. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"